My first thought was a reversed scope base as well. Or something wrong with the scope base; I had a CZ 527 I couldn't get on paper back in the day, and it was because one of the scope rings was keyed, and I had that one in the wrong spot and the key had the ring popped up out of place.
@Ty21 If you look at a ballistics table for a subsonic 22LR, the dope is almost the same at 25 yds as it is at 50 yds; with a 50-yd zero and subsonic ammo, the bullet usually crosses line of sight around 21-24 yards, then travels above LOS until dropping back below at 50 yards. This isn't the issue.